Intensive anthropogenic activity has strongly impacted the fauna of Pryingulsky RLP. Cattle pasture, recreational loads, amateur fishery, poaching in the area of Sofiivske water reservoir, steppe and forest fire deplete the fauna of RLP, which, however, is much better preserved than in the bordering agrolandscapes.

The list of rare and threatened species being under protection of the State includes Southern Festoon (Zerynthia polyxena), Old World Swallowtail (Papilio machaon), Mammoth Wasp (Scolia maculata), Carpenter Bee (Xylocopa valga), Large Whip Snake (Coluber jugularis), Long-legged Buzzard (Buteo rufinus), Stoat or Ermine (Mustela erminea), Steppe Polecat (Mustela eversmanii) etc.